Format: MS WORD | Chapter: 1-5 | Pages: 65-80 | .
INFLUENCE OF GALLIC ACID ON Α-AMYLASE AND Α-GLUCOSIDASE INHIBITORY AND ANTIOXIDANT PROPERTIES OF ACARBOSE
ABSTRACT
Type 2 diabetes mellitus (T2DM) is a chronic progressive disease that has continued to be a global heath and economic burden. Acarbose is an antidiabetic drug, which acts by inhibiting alpha amylase and alpha glucosidase; while gallic acid is a simple phenolic acid that is widespread in plant foods and beverages such as tea and wine. This study therefore, sought to investigate the influence of gallic acid on α-amylase and α-glucosidase inhibitory and antioxidant properties of acarbose (in vitro). Aqueous solution of acarbose and gallic acid were prepared to a final concentration of 25μM each. Thereafter, mixtures of the samples (50% acarbose + 50% gallic acid; 75% acarbose + 25% gallic acid; 25% acarbose + 75% gallic acid) were prepared. The results showed that the combination of 50% acarbose and 50% gallic acid showed the highest α-glucosidase inhibitory effect, while 75% acarbose + 25% gallic acid showed highest α- amylase inhibitory effect. Furthermore, all the samples caused the inhibition of Fe2+-induced lipid peroxidation (in vitro) in rat pancreatic tissue homogenate, with the combination of 50% acarbose and 50% gallic acid causing the highest reduction in the malondialdehyde content. In addition, all the samples showed antioxidant properties (ferric reducing property, 2, 2′-azino-bis (-3-ethylbenzthiazoline-6-sulphonate (ABTS*) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging abilities, and Fe2+ chelating ability). Therefore, the combinations of gallic acid with acarbose could be employed in the management of T2DM with the comparative advantage of possible reduction of the side effects of acarbose; nevertheless the combination of 50% acarbose and 50% gallic acid seems the best combinatory therapy for the management of
type 2 diabetes mellitus.
HERE IS WHAT OUR HAPPY CLIENTS ARE SAYING
I actually googled and saw about projectmaster.com.ng, copied the number and contacted them through WhatsApp to ask for the availability of the material and to my luck they have it. So there was a delay with the project due to the covid19 pandemic. I was really scared before making the payment cause I’ve been scammed twice, they attended so well to me and that made me trust the process and made the payment and provided them with proof, I got my material in less than 10minutes.
I received excellent customer support, and quickly. Thank you so much!
I love your service! I would recommend projectmaster.com.ng to anyone. What a great find!
I am aware of online scam but because of the hectic nature of writing project, I decided to try sourcing for help online and to my surprise, projectmaster.com.ng gave complete project in less than 5 minutes. I could not believe it sincerely. Thank you so much guys.